Journal: Applied and Environmental Microbiology

Article Title: Nisin H Is a New Nisin Variant Produced by the Gut-Derived Strain Streptococcus hyointestinalis DPC6484

doi: 10.1128/AEM.00212-15

Figure Lengend Snippet: Bacterial strains and culture conditions

Article Snippet: Cross immunity of Streptococcus hyointestinalis DPC6484 to other nisin-producing strains The activities of purified nisin A and nisin H peptides against Escherichia coli DPC6912, Bacillus cereus 9139, L. delbrueckii subsp. bulgaricus LMG6901, Lactococcus lactis subsp. cremoris HP, Enterococcus faecalis 6307, S. agalactiae ATCC 13813, S. agalactiae DPC5338, Streptococcus bovis DPC6491, S. gallolyticus DPC6501, Listeria innocua DPC3572, Listeria monocytogenes 1042, S. aureus ATCC 25923, S. aureus DPC5245, S. hyointestinalis DPC6484, and S. uberis strain 42 were assessed ( ).

Techniques: Incubation

Journal: Applied and Environmental Microbiology

Article Title: Nisin H Is a New Nisin Variant Produced by the Gut-Derived Strain Streptococcus hyointestinalis DPC6484

doi: 10.1128/AEM.00212-15

Figure Lengend Snippet: Spectra of inhibition of purified nisin A and nisin H peptides against a range of strains

Article Snippet: Cross immunity of Streptococcus hyointestinalis DPC6484 to other nisin-producing strains The activities of purified nisin A and nisin H peptides against Escherichia coli DPC6912, Bacillus cereus 9139, L. delbrueckii subsp. bulgaricus LMG6901, Lactococcus lactis subsp. cremoris HP, Enterococcus faecalis 6307, S. agalactiae ATCC 13813, S. agalactiae DPC5338, Streptococcus bovis DPC6491, S. gallolyticus DPC6501, Listeria innocua DPC3572, Listeria monocytogenes 1042, S. aureus ATCC 25923, S. aureus DPC5245, S. hyointestinalis DPC6484, and S. uberis strain 42 were assessed ( ).

Techniques: Inhibition, Purification

Journal: Applied and Environmental Microbiology

Article Title: Nisin H Is a New Nisin Variant Produced by the Gut-Derived Strain Streptococcus hyointestinalis DPC6484

doi: 10.1128/AEM.00212-15

Figure Lengend Snippet: Purification of nisin H from Streptococcus hyointestinalis DPC6484 grown in TSB broth. (A) RP-HPLC chromatogram; (B) MALDI-TOF MS of the active fraction; (C) zone of inhibition of an aliquot of the HPLC fraction on an Lactobacillus delbrueckii subsp. bulgaricus LMG6901 indicator plate.

Article Snippet: Cross immunity of Streptococcus hyointestinalis DPC6484 to other nisin-producing strains The activities of purified nisin A and nisin H peptides against Escherichia coli DPC6912, Bacillus cereus 9139, L. delbrueckii subsp. bulgaricus LMG6901, Lactococcus lactis subsp. cremoris HP, Enterococcus faecalis 6307, S. agalactiae ATCC 13813, S. agalactiae DPC5338, Streptococcus bovis DPC6491, S. gallolyticus DPC6501, Listeria innocua DPC3572, Listeria monocytogenes 1042, S. aureus ATCC 25923, S. aureus DPC5245, S. hyointestinalis DPC6484, and S. uberis strain 42 were assessed ( ).

Techniques: Purification, Inhibition

Journal: Applied and Environmental Microbiology

Article Title: Nisin H Is a New Nisin Variant Produced by the Gut-Derived Strain Streptococcus hyointestinalis DPC6484

doi: 10.1128/AEM.00212-15

Figure Lengend Snippet: Cross immunity of Streptococcus hyointestinalis DPC6484 to other nisin-producing strains

Article Snippet: Cross immunity of Streptococcus hyointestinalis DPC6484 to other nisin-producing strains The activities of purified nisin A and nisin H peptides against Escherichia coli DPC6912, Bacillus cereus 9139, L. delbrueckii subsp. bulgaricus LMG6901, Lactococcus lactis subsp. cremoris HP, Enterococcus faecalis 6307, S. agalactiae ATCC 13813, S. agalactiae DPC5338, Streptococcus bovis DPC6491, S. gallolyticus DPC6501, Listeria innocua DPC3572, Listeria monocytogenes 1042, S. aureus ATCC 25923, S. aureus DPC5245, S. hyointestinalis DPC6484, and S. uberis strain 42 were assessed ( ).

Techniques: Inhibition

Journal: Applied and Environmental Microbiology

Article Title: Nisin H Is a New Nisin Variant Produced by the Gut-Derived Strain Streptococcus hyointestinalis DPC6484

doi: 10.1128/AEM.00212-15

Figure Lengend Snippet: (A) PFGE macrorestriction patterns of Streptococcus hyointestinalis strains restricted with SmaI. Lane 1, S. hyointestinalis DPC6484; lane 2, S. hyointestinalis LMG14579; lane 3, S. hyointestinalis LMG14581; lane 4, S. hyointestinalis LMG14582; lane 5, S. hyointestinalis LMG14583; lane 6, S. hyointestinalis LMG14585; lane 7, S. hyointestinalis LMG14586; lane 8, S. hyointestinalis LMG14587. (B) (i) PCR amplification of strains of S. hyointestinalis template DNA with nshT-, nshH-, nshF-, and nshR-specific primers. Lanes correspond to those in panel A. (ii) Comparison of antimicrobial activities of S. hyointesinalis strains DPC6484 (H), LMG14579, LMG14581, LMG14582, LMG14583, LMG14585, LMG14586, and LMG14587 against the indicator strain, L. delbrueckii subsp. bulgaricus LMG6901. Wells are labeled with H (for nisin H) and with the last two digits of the strain designation for the other strains.

Article Snippet: Cross immunity of Streptococcus hyointestinalis DPC6484 to other nisin-producing strains The activities of purified nisin A and nisin H peptides against Escherichia coli DPC6912, Bacillus cereus 9139, L. delbrueckii subsp. bulgaricus LMG6901, Lactococcus lactis subsp. cremoris HP, Enterococcus faecalis 6307, S. agalactiae ATCC 13813, S. agalactiae DPC5338, Streptococcus bovis DPC6491, S. gallolyticus DPC6501, Listeria innocua DPC3572, Listeria monocytogenes 1042, S. aureus ATCC 25923, S. aureus DPC5245, S. hyointestinalis DPC6484, and S. uberis strain 42 were assessed ( ).

Techniques: Amplification, Comparison, Labeling